Antagonism of Trichoderma sp. against Colletotrichum capsici: In Vitro Testing as a Biological Control Strategy for Anthracnose Disease in Chili Peppers

Authors

  • Triska Wahyuni Dawit Universitas Negeri Gorontalo Author
  • Ani M. Hasan Universitas Negeri Gorontalo Author
  • Jusna Ahmad Universitas Negeri Gorontalo Author
  • Yuliana Retnowati Universitas Negeri Gorontalo Author
  • Febriyanti Febriyanti Universitas Negeri Gorontalo Author

DOI:

https://doi.org/10.65310/dvr6m616

Keywords:

In Vitro, Cayenne Pepper, Colletotrichum Capsici, Trichoderma sp., Capsicum frutescens.

Abstract

Anthracnose disease is a plant disease caused by the fungus Colletotrichum capsici, the disease is one of the problems in the cultivation of cayenne pepper (Capsicum frutescens). This study aims to test the antagonistic ability of Trichoderma sp. against the fungus Colletotrichum capsici and observe the morphological changes in Colletotrichum capsici colonies exposed to Trichoderma sp in vitro on cayenne pepper plants Capsicum frutescens. The test method was carried out using the dual culture method to determine the percentage of inhibition by studying the interaction between Trichoderma sp. and Colletotrichum capsici colonies on Potato Dextrose Agar culture media. The results showed that Trichoderma sp. fungi can compete for space and resources against Colletotrichum capsici fungi macroscopically by controlling space and resources, and microscopically by producing antibiotics (antimicrobials) that can inhibit the growth of Colletotrichum capsici. The urgency of this research is the need for safe and environmentally friendly disease control efforts. One method that has been widely developed is by utilizing biological agents, such as Trichoderma sp. which is able to inhibit the growth of the pathogen Colletotrichum capsici.

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Published

2025-12-28

How to Cite

Antagonism of Trichoderma sp. against Colletotrichum capsici: In Vitro Testing as a Biological Control Strategy for Anthracnose Disease in Chili Peppers. (2025). Journal of Science, Technology, and Innovation, 1(2), 201-211. https://doi.org/10.65310/dvr6m616