The Effect of Diazinon Pesticide Administration on Elevated SGPT (Serum Glutamic-Pyruvic Transaminase) Levels in Male Mice
DOI:
https://doi.org/10.65310/5jtdxx14Keywords:
Diazinon, SGPT, Hepatotoxicity, Liver, Mus Musculus.Abstract
This study aimed to evaluate the effect of diazinon pesticide exposure on serum glutamic pyruvic transaminase (SGPT) levels in male mice as a biomarker of liver injury. A true experimental design with a posttest-only control group approach was implemented using 25 male mice (Mus musculus) randomly assigned into one control group and four treatment groups with graded diazinon doses. The exposure was administered orally for 10 consecutive days under controlled laboratory conditions. Blood samples were collected to measure SGPT levels using a kinetic spectrophotometric method. Statistical analysis was conducted using one-way ANOVA followed by post hoc testing to determine intergroup differences. The findings demonstrated a significant elevation of SGPT levels in all treatment groups compared to the control (p < 0.05), indicating hepatocellular damage. Further analysis revealed significant differences between treatment groups, suggesting a dose-related toxic effect. These results confirm that diazinon exposure induces liver dysfunction and enzymatic alterations, emphasizing its hepatotoxic potential.
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Abasa, S., & Ishak, P. (2022). Aktivitas hepatoprotektor Ekstrak Etanol Kulit Buah Naga Merah (Hylocereus polyrhizus) Terhadap hepatotoksisitas Akut Paracetamol Pada Tikus (Rattus norvegicus). Pharmacology And Pharmacy Scientific Journals, 1(1), 11-19. https://doi.org/10.51577/papsjournals.v1i1.304
Alfhadillah, R., Astuti, P., Rahmayanti, S., & Novianry, V. (2026). Analysis of Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT) Levels in Rats Given Jamu Cekok Decoction. Biology, Medicine, & Natural Product Chemistry, 15(1), 189-198. https://doi.org/10.14421/biomedich.2026.151.189-198
Cassaret, L. J., & Doull, J. (1975). Toxicology: The basic science of poisons. Macmillan Publishing.
Djojosumarto, P. (2008). Pestisida dan aplikasinya. Agro Media Pustaka.
Donatus, A. I. (2002). Toksikologi dasar. Laboratorium Farmakologi dan Toksikologi Fakultas Farmasi Universitas Gadjah Mada.
Dyce, K. M., Sack, W. O., & Wensing, C. J. G. (2002). Textbook of veterinary anatomy (Ed. ke-3). Elsevier.
Elpira, W. A., Pusmarani, J., & Baco, J. (2025). Aktivitas Hepatoprotektor Ekstrak Metanol Daun Kluwih (Artocarpus camansi) terhadap Kadar SGOT-SGPT Plasma Darah Tikus (Rattus norvegicus) yang di Induksi Parasetamol. Jurnal Pharmacia Mandala Waluya, 4(2), 104-116. https://doi.org/10.54883/jpmw.v4i2.264
Ferara, E., Iskandar, A. R., & Lubis, A. R. B. (2025). Pengaruh Pemberian Ekstrak Daun Sirsak (Annona Muricata L.) Terhadap Fungsi Hati Dan Gambaran Histopatologi Hati Tikus Putih Galur Wistar Jantan Yang Mengalami Diabetes Melitus. Jurnal Penelitian Pendidikan, Psikologi Dan Kesehatan (J-P3K), 6(2), 618-628. https://doi.org/10.51849/j-p3k.v6i2.744
Firdaus, J., Febianti, Z., Hidayat, M. R. F., & Sakinah, E. N. (2022). Efek Neem Gum (Azadiracthta indica) terhadap kadar SGOT SGPT tikus Wistar yang diinduksi Diazinon. Journal of Nutrition College, 11(3), 258-263. https://doi.org/10.14710/jnc.v11i3.33384
Fristiohady, A., Malik, F., Wahyuni, W., Idrus, L. S., Saranani, M. A. A. F., Sadarun, B., & Kardin, L. (2024). Aktivitas Hepatoprotektor Ekstrak Etanol Spons Sinularia sp. Pada Mencit (Mus musculus) Yang Diinduksi Karbon Tetraklorida (CCl4). Lansau: Jurnal Ilmu Kefarmasian, 2(1), 80-87. https://doi.org/10.33772/lansau.v2i1.27
Guyton, A. C., & Hall, J. E. (2006). Buku ajar fisiologi kedokteran (L. Y. R. Dr, H. H. dr, A. N. dr, & N. W. dr, Ed.; Ed. ke-9). EGC.
Harada, T., Akiko, E., Gary, A. B., & Robert, R. M. (1999). Liver and gallblader. Dalam R. R. Maronpot, A. B. Gary, & G. W. Beth (Ed.), Pathology of the mouse (hlm. 119–171). Cache River Press.
Harahap, H. (2024). Alternate Day Fasting terhadap Berat Badan, dan Enzim Transaminase pada Tikus Putih Galur Sprague Dawley. Scientific Of Environmental Health and Diseases, 4(2), 34-38. https://doi.org/10.22437/esehad.v5i1.35373
Husniyyah, E. A., Simamora, D., & Widyaningsih, I. (2023). Profil SGPT pada Tikus Wistar Diet Tinggi Lemak yang diberikan Ekstrak Daun Ashitaba. Briliant: Jurnal Riset dan Konseptual, 8(4), 1041-1048. https://doi.org/10.28926/briliant.v8i4.1413
Idrus, L. S., Fristiohady, A., Arfan, A., Jannah, S. R. N., Anwar, I., Sida, N. A., ... & Yodha, A. W. M. (2023). Potensi Hepatoprotektor dan Antioksidan dari Rimpang Olae (Etlingera calophrys (K. Schum) AD Poulsen). Jurnal Mandala Pharmacon Indonesia, 9(2), 475-483. https://doi.org/10.35311/jmpi.v9i2.409
Lubis, W. R., & Syukriah, S. (2025). The Effect of Giving Ethanol Extract of Palm Fruit (Arenga pinnata Merr.) on Morphology and Liver Function of White Rats (Rattus norvegicus L.) Induced by Carbon Tetrachloroide. Jurnal Biologi Tropis, 25(2), 1269-1274. https://doi.org/10.29303/jbt.v25i2.8731
Makrufah, M., & Rohmah, J. (2025). Toxicity of White Turi Leaf Extract on Liver Function in Mice: Toksisitas Ekstrak Daun Turi Putih pada Fungsi Hati Mencit. Indonesian Journal on Health Science and Medicine, 2(2), 10-21070. https://doi.org/10.21070/ijhsm.v2i2.246
Mangkoewidjojo, S., & Smith, J. B. (1988). Pemeliharaan, pembiakan dan penggunaan hewan percobaan di daerah tropis. Universitas Indonesia.
Muawanah, M., Rasyid, N. Q., & Nurhikmah, N. (2025). Pengaruh Masa Kerja Petani Pengguna Pestisida Terhadap Kadar SGPT (Serum Glutamic Pyruvic Transaminase). MEDIKA TRADA, 6(2), 134-139. https://doi.org/10.59485/jtemp.v6i2.158
Munasari, D., Fristiohady, A., Malik, F., & Sadarun, B. (2024). Aktivitas Hepatoprotektor Ekstrak Etanol Karang Lunak Lobophytum sp. Pada Mencit Putih yang Diinduksi CCL4. Lansau: Jurnal Ilmu Kefarmasian, 2(1), 62-70. https://doi.org/10.33772/lansau.v2i1.25
Nadhifah, D., Mufidah, H., & Muflihah, A. I. (2025). Evaluation of Liver Function Through SGOT and SGPT Quantification in Rats Administered Polyethylene Terephthalate Microplastics. Journal of Medical Laboratory in Infectious and Degenerative Diseases, 3(1), 1-9. https://doi.org/10.36858/jmid.v3i1.29
Nazir, M. (2003). Metode penelitian. Ghalia Indonesia.
Notoatmodjo, S. (2010). Promosi kesehatan: Teori & aplikasi. Rineka Cipta.
Prasuma, G. S., Qotrunnada, E. N., & Charisma, S. L. (2023). Uji Aktivitas Hepatoprotektor Ekstrak Etanol Herba Tapak Liman (Elephantopus scaber L.) terhadap Tikus Jantan Galur Wistar yang Diinduksi Isoniazid. PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 183-188. https://doi.org/10.30595/pharmacy.v20i2.20317
Pratiwi, P. R., Herdwiani, W., & Wijayanti, T. (2023). Uji Aktivitas Hepatoprotektor Ekstrak Etanol dan Fraksi N-Heksana, Etil Asetat, dan Air Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr.) pada Tikus Jantan Galur Wistar. Jurnal Penelitian Kesehatan" SUARA FORIKES"(Journal of Health Research" Forikes Voice"), 14(1), 77-82. http://dx.doi.org/10.33846/sf14116
Price, S. A., & Wilson, L. M. (1984). Patofisiologi: Konsep klinik proses-proses penyakit bagian I (A. Dharmawan, Terj.). EGC.
Sahwahita, M. N., Astuti, T. D., & Ratih, W. U. (2025). Pemberian Ekstrak Methanol Trichosanthes cucumerina terhadap Kadar SGOT dan SGPT pada Tikus Ulkus Diabetikum. Jurnal Kesehatan Perintis, 12(1), 8-16. https://doi.org/10.33653/4k6yb202
Sandhiutami, N. M. D., Dewi, R. S., Khairani, S., & Widyadari, S. A. M. (2022). Evaluasi Keamanan dari Pengembangan Formula Nanopartikel Kurkumin pada Mencit dan Potensi Antioksidan In-VitroEvaluasi Keamanan dari Pengembangan Formula Nanopartikel Kurkumin pada Mencit dan Potensi Antioksidan In-Vitro. Jurnal Ilmu Kefarmasian Indonesia, 20(1), 63-72. https://doi.org/10.35814/jifi.v20i1.1187
Simanullang, G., Nabila, N. A., Rahayyu, A. M., Wardani, I. K., & Hammami, A. (2025). Uji Aktivitas Hepatoprotektor Tablet Curcuma® terhadap Kadar SGPT dan SGOT pada Tikus yang Diinduksi Isoniazid. Jurnal Mandala Pharmacon Indonesia, 11(2), 648-657. https://doi.org/10.35311/jmpi.v11i2.848
Sudarmo, S. (2007). Pestisida. Kanisius.
Sudiono, J. B., Kurniadhi, Hendrawan, A., & Djinantoro, B. (2003). Ilmu patologi. EGC.
Sulaeman, A., Agustian, S., Susilawati, E., Muhsinin, S., Kaniawati, M., & Feliana, M. Efek Kombinasi Ekstrak Etanol Rimpang Kunyit (Curcuma longa L.) dan Daun Pegagan (Centella asiatica (L.) Urb) terhadap Ekspresi Gen eNOS pada Jaringan Hati Tikus Obes. Jurnal Pharmascience, 12(2), 542-557. https://dx.doi.org/10.20527/jps.v12i2.23813
Syahfitri, F. R., Syukriah, S., & Febriani, H. (2025). Pengaruh Ekstrak Daun Salam (Syzygium Polyanthum) Terhadap Hati Tikus Putih (Rattus Norvegicus) Yang Diinduksi Kadmium Klorida. Bioma: Jurnal Biologi Makassar, 10(1), 79-91. https://doi.org/10.20956/bioma.v10i1.41623
Ukratalo, A. M. (2024). Profil Kadar Enzim Serum Glutamat Piruvat Transaminase (SGPT) dan Serum Glutamat Oksaloasetat Transaminase (SGOT) Mencit Model Diabetes Melitus Pasca Pemberian Ekstrak Kulit Batang Cinnamomum burmanii. Jurnal Anestesi, 2(4), 382-391. https://doi.org/10.59680/anestesi.v2i4.1454
Widmann, F. K. (1995). Tinjauan klinis atas hasil pemeriksaan laboratorium (Ed. ke-9). EGC.
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